Authors: Shy-Shin Chang, Hsung-Ling Hsu, Ju-Chien Cheng, Ching-Ping Tseng
DOI: 10.1371/journal.pone.0020303
Abstract Summary
Researchers developed a novel primer extension-PCR method that eliminates bacterial DNA contamination in PCR reagents without compromising efficiency. The technique uses fusion probes to selectively amplify target bacteria while ignoring contaminants, detecting as little as 10-100 fg of DNA. Combined with real-time and high-resolution melting analysis, it enables accurate bacterial identification in clinical settings.
Why Brain? ðŸ§
Novel PCR method detects trace bacteria without removing DNA contaminants from reagents, enabling accurate identification of low-abundance pathogens in clinical samples using melting curve analysis.
License: CC BY.
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